Title: Microsoft Word - Stanford Fortessa HTS User Guide.docx Author: lovelace Created Date: 8/14/2013 10:44:41 PM

LSR Fortessa . User Guide . Ragon Institute of Massachusetts General (printed version on back cover of User Guide Binder) 8. Optimizing Setup of the LSR2 system Exporting, Experiment Templates, Transferring files . 12. HTS (3L and 5L only) 13. Troubleshooting . Baseline Correction and …

This will warm up the lasers and pressurize the fluidics. 2. Switch on the Computer and login to the DiVa software. Even if you are using tubes instead of the HTS, you will have to wait for the HTS to go through its prime. 3. Check the fluidics.

Bd fortessa hts user guide

This document describes how to set up and operate the HTS. It also contains a description of BD FACSDiva software features specific to the HTS. As a frequently referenced high-parameter cell analyzers, the BD LSRFortessa brand provides power, performance and consistency for your research. Designed to be affordable and expandable, BD LSRFortessa cell analyzers have the flexibility to support the growing needs of multicolor flow cytometry assays. Sony user guide. Sony SH800 cell sorter operators guide. BD Accuri C6 flow cytometer. Instrument manual.

http://stratedigm.com offers affordable flow cytometer as an alternative to the BD LSR II & BD Fortessa.Call( 408) 512-3901 to learn more about our flow cyto

Remove the HTS safety cover. Locate and loosen the positioning screw that secures the HTS to the cytometer support bracket (Figure 4-17 on page 133). The BD LSRFortessa™ X-20 fits most flow cytometry analyzer needs, and can be ordered with up to 5 lasers-most commonly, blue, red, violet, yellow-green, and UV-which provides flexibility in laser wavelengths. This allows researchers to optimize assay design by selecting the latest fluorescent dyes and antibodies in experimental protocols.

Users who continue to block the SIP through neglectful sample preparation may have their usage privileges withdrawn. The Fortessa has a 405nm, 488nm, 561nm and 633nm laser setup with the ability to detect signals in 16 channels. Before designing an experiment or purchasing an antibody, please ensure it is compatible with this instrument.

BD FACSDiva Software Basic Experiment Guide Please note, this guide is not intended to take the place of in-person training but is to be used as a reference as needed after completing training with Unified Flow Core staff. GETTING STARTED 1. If you are the first user of the day: Boot up the computer and log on to Windows. Wait 10 seconds, then BD LSR II Quick User Guide Page 4 13. When you are finished and if you are not the last booked user for the day, log out of your centre folder by selecting File > Log Out. The screen should look like this ready for the next user. NB PLEASE DO NOT LOG OUT OF THE FACS DIVA SOFTWARE AS THIS MAY CAUSE CONNECTIVITY PROBLEMS FOR THE NEXT USER.

Switch on the Computer and login to the DiVa software.
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Acq Instrument: LSR Fortessa HTS. Date:_31 Mar 2015__. By:____Teia___.

3. Check the fluidics. Fludics Default cytometer configuration for the LSR Fortessa analyzer: For more information click the following links: BD LSR Fortessa user manual.
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This guide contains instructions for using BD FACSDiva ™ software version 6.0 and later with BD LSR II flow cytometers equipped with the BD ™ High Throughput Sampler (HTS) option. Most of the features for running plate-based experiments on the BD ™ HTS option are located in the Plate window.

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BD LSR Fortessa Flow Cytometry Analyzer equipped with HTS (High Throughput Sampler) BD FACSDiva HTS quick reference guide · HTS quick reference

The HTS supports standard 96 (u bottom plates best) and 384 well plates. It is operated using DiVA allowing users to define customized delivery protocols, mixing, wash and analysis parameters The BD LSRFortessa™ X-20 fits most flow cytometry analyzer needs, and can be ordered with up to 5 lasers-most commonly, blue, red, violet, yellow-green, and UV-which provides flexibility in laser wavelengths. This allows researchers to optimize assay design by selecting the latest fluorescent dyes and antibodies in experimental protocols.